Raman Spectroscopy Fundamentals


Raman spectroscopy provides vibrational, rotational and low frequency modes detection and is a very useful tool to investigate a molecule’s internal structure. In this context, this course is designed to cover the theoretical background of the Raman spectroscopy and to discuss a variety of example applications, through tutorial exercises and laboratory practice, complemented by lectures. More specifically, students will be taught Raman spectroscopy basic theory, principles and instrumentation, as well as to collect and interpret spectral data, covering a long range of samples/applications, through mapping and imaging video demonstration, in order potential users to be educated in obtaining and interpreting optimal high-quality spectral data, carried out on a Raman spectrometer.


  • Historical introduction of the Raman spectroscopy
  • Theory of Raman spectroscopy on molecules and crystals
  • Raman basic theory brief review
  • Raman spectroscopy option
  • Parameters selection and comparison to IR spectroscopy
  • Raman instrumentation (dispersive and FT)
  • Practical laboratory courses on Raman spectroscopy
  • Applications (carbon-based materials, polymers, pigments, nanoparticles etc.)
  • Spectral interpretation
  • Library searches
  • Mapping and Imaging (video demonstration)

Learning Outcomes

Students will learn or/and improve their ability to apply Raman spectroscopy to their samples, to interpret the respective spectral data in order to expand the scope of their applications.


1. Wang, L.; Mizaikoff, B.; Kranz, C. “Quantification of Sugar Mixtures with Near-infrared Raman Spectroscopy and Multivariate Data Analysis A Quantitative Analysis Laboratory Experiment” J. Chem. Ed. 2009, 86, 1322.
2. Vickers, T.J.; Pecha, J.; Mann, C.K. “Raman spectroscopy with a fiber-optic probe and multichannel detection” J. Chem. Ed. 2001, 78, 1674.
3. Long, D.A., Raman Spectroscopy, McGraw-Hill, Inc., New York, 1977.
4. Harris, D. C.; Bertolucci, M. D.; Symmetry and Spectroscopy: an introduction to vibrational and electronic spectroscopy, Dover Publications, Inc., New York, 1989.
5. Szymanski, H. A., Raman Spectroscopy: Theory and Practice, Plenum Press, New York, 1967.
6. McCreery, R. L., Raman Spectroscopy for Chemical Analysis, John Wiley & Sons, Inc., New York, 2000.

External Evaluator

Prof. Xaniotakis (University of Crete)

Responsible Academic

Dr Minas Stylianakis (TEI of Crete)

Awarded ECTS